Journal: Lipids in Health and Disease

Article Title: C-reactive protein/oxidised low-density lipoprotein/β2-glycoprotein I complex promotes atherosclerosis in diabetic BALB/c mice via p38mitogen-activated protein kinase signal pathway

doi: 10.1186/1476-511X-12-42

Figure Lengend Snippet: Effect of CRP / oxLDL / β2 - GPI on infiltration of inflammatory cells in the aortas. Aortas were removed, paraffin-embedded, deparaffinated, underwent antigen retrieval, blocked, and then incubated with primary antibodies (Mac 1:200 or α- SMA 1:100 or CD3 1:100) at 4°C overnight. Biotinylated secondary antibody (1:200) was added, followed by HRP-labeled streptavidin incubation. Infiltrations of macrophages, SMCs and T cells in the aortas were quantified as the percentage of the luminal surface covered by positive cells to the plaque area using Image Pro-Plus 6.0. Results are shown as means ± SEM. A : Infiltration of SMCs in the aortas, 400× magnification. B : Infiltration of macrophages in the aortas, 400× magnification. C : Infiltration of T cells in aortas, 400× magnification. D : Positive expressions of SMA, MAC and CD3 (n=4), expressed as the percentage to plaque area. * P <0.05, compared with the CRP/oxLDL/β2GPI group, † P < 0.05, compared with the PBS group.

Article Snippet: Mice anti-α-smooth muscle actin (SMA, BM0002) antibody, rabbit anti-CD3 (BA0429) antibody were purchased from Boster, Wuhan, China.

Techniques: Incubation, Labeling

Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression of Wnt3a, BMP2 and β-catenin nuclear proteins in NSCs after HBO treatment by Western blotting. (A) Expression of Wnt3a and BMP2 protein: HBO treatment up-regulated the expression of Wnt3a and BMP2 proteins. (B) Expression of β-catenin nuclear and cytoplasmic proteins: HBO up-regulated the expression of β-catenin nuclear protein; however, the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the CON group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test; c p < 0.05 vs. the NBH group, ANOVA test; d p < 0.05 vs. the HIBH group, ANOVA test. NSCs: neural stem cells; HBO: hyperbaric oxygen; CON: control; NBH: normal brain tissue extracts; HIBH: hypoxic-ischemic brain damage tissue extracts; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Cell Transplantation

Article Title: HBO Promotes the Differentiation of Neural Stem Cells via Interactions Between the Wnt3/β-Catenin and BMP2 Signaling Pathways

doi: 10.1177/0963689719883578

Figure Lengend Snippet: Expression, after HBO treatment, of Wnt3a, BMP2 and β-catenin nuclear proteins in the presence of sFRP2/3 was determined by Western blotting. (A) Expression of Wnt3a and BMP2 proteins: sFRP2/3 reduced the expression of Wnt3a and BMP2 proteins after HBO treatment. (B) Expression of β-catenin nuclear and cytoplasmic proteins: sFRP2/3 reduced the expression of β-catenin nuclear protein after HBO treatment; the expression of β-catenin cytoplasmic protein was not affected. (C and D) Each bar represents mean±SD, n = 3. a p < 0.05 vs. the HIBD+HBO group, ANOVA test; b p < 0.05 vs. the HBO group, ANOVA test. HBO: hyperbaric oxygen; CON: control; HIBD: hypoxic-ischemic brain damage; sFRP2/3: secreted Frizzled-related protein 2 and 3; BMP2: bone morphogenetic protein.

Article Snippet: The following primary antibodies were used: Rabbit mAb active Wnt3 (1:100; SC-28824, Santa Cruz, Biotechnology, Inc., Dallas, TX, USA), rabbit mAb active β-catenin (1:100; BA0426, Wuhan Boster Biological Technology, Ltd.), rabbit mAb active BMP2 (1:100; PB0727, Wuhan Boster Biological Technology, Ltd.), mouse mAb β-actin (1:200, BM0626, Wuhan Boster Biological Technology, Ltd.) and mouse mAb active PCNA (1:1000; 610664, BD Biosciences, San Jose, CA, USA).

Techniques: Expressing, Western Blot, Control

Journal: Frontiers in Pharmacology

Article Title: Feiyiliu Mixture sensitizes EGFR Del19/T790M/C797S mutant non-small cell lung cancer to osimertinib by attenuating the PRC1/Wnt/EGFR pathway

doi: 10.3389/fphar.2023.1093017

Figure Lengend Snippet: FYLM combined with osimertinib regulated PRC1/Wnt pathway in EGFR-Del19/T790M/C797S LLC tumor-bearing mice. (A) Detection of β-catenin expression in tumor tissues by immunohistochemistry. (B,D) PRC1, β-catenin, c-Myc and c-Jun proteins were detected by western blotting. (C,E–G) Quantitative analysis of PRC1, β-catenin, c-Myc and c-Jun protein levels. ** p < 0.01 and *** p < 0.001 vs. Control; # p < 0.05, ## p < 0.01 and ### p < 0.001 vs. Osi; △ p < 0.05 and △△△ p < 0.001 vs. Osi + FYLM (24 g/kg). Osi, osimertinib. All data are shown as mean ± SD.

Article Snippet: The primary antibodies were as follows: EGFR (#4267, Cell Signaling Technology, 1:1000), p-EGFR (#3777s, Cell Signaling Technology, 1:1000), Cyclin B1 (#4138, Cell Signaling Technology, 1:1000), Bcl-2 (#3498, Cell Signaling Technology, 1:1000), Cleaved Caspase-3 (#9661, Cell Signaling Technology, 1:1000), PRC1 (BM3910, BOSTER, Wuhan, China, 1:1000), β-catenin (BA0426, BOSTER, Wuhan, China, 1:1000), c-Myc (PB9092, BOSTER, Wuhan, China, 1:1000), c-Jun (#9165, Cell Signaling Technology, 1:1000), Akt (ab179463, Abcam, 1:10000), p-Akt (ab192623, Abcam, 1:1000), β-actin (BA2305, BOSTER, Wuhan, China, 1:5000), GAPDH (A00227-1, BOSTER, Wuhan, China, 1:1000).

Techniques: Expressing, Immunohistochemistry, Western Blot